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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
23/03/2018 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
OLINDA, R. G.; MAIA, L. A.; FRADE, M. T. S.; SOARES, M. P.; BARROS, S. S.; DRIEMEIER, D.; RIET-CORREA, F.; DANTAS, A. F. M. |
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Afiliación : |
Programa de Pós-graduação em Medicina Veterinária, Hospital Veterinário, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG); Programa de Pós-graduação em Medicina Veterinária, Hospital Veterinário, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG); Programa de Pós-graduação em Medicina Veterinária, Hospital Veterinário, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG); Laboratório Regional de Diagnóstico, Faculdade de Veterinária (FV), Universidade Federal de Pelotas (UFPel); Laboratório Regional de Diagnóstico, Faculdade de Veterinária (FV), Universidade Federal de Pelotas (UFPel); Departamento de Patologia Clínica Veterinária, Faculdade de Veteriná- ria, Universidade Federal do Rio Grande do Sul (UFRGS),; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Programa de Pós-graduação em Medicina Veterinária, Hospital Veterinário, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG); Programa de Pós-graduação em Medicina Veterinária, Hospital Veterinário, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG). |
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Título : |
Degenerative axonopathy associated with copper deficiency in pigs. (Axonopatia degenerativa associada com deficiência de cobre em suínos). |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Pesquisa Veterinária Brasileira, Rio de Janeiro v. 37, n. 9, p. 911-915, setembro. 2017. |
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DOI : |
10.1590/S0100-736X2017000900002 |
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Idioma : |
Inglés |
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Notas : |
Article history: 1 Received on May 29, 2016 // Accepted for publication on November 11, 2016. |
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Contenido : |
Abstract: The epidemiological, clinic and morphological (pathological and ultrastructural) aspects of four outbreaks of copper deficiency affecting 21- to 90-day-old pigs in the Northeast region of Brazil are reported. Clinical signs began with paraparesis and ataxia and progressed to flaccid or spastic paralysis of the pelvic and thoracic limbs, followed by sternal and/or lateral recumbence. In addition, some animals showed dog-sitting position and intention tremors. The clinical manifestation period was 5-20 days. Significant gross lesions were not observed; however, microscopically, symmetrical degeneration of the white matter with ballooned myelin sheaths containing occasional macrophages was observed, mainly in the spinal cord. Two pigs presented with necrosis ad loss of Purkinje cells and ectopic Purkinje cells in the granular layer and cerebellar white matter. A ultrastructural analysis showed different degrees of damage of myelinated axons in the spinal segments, including an absence of the axoplasm structures with only axonal residues remaining. The myelin sheaths were degenerated and often collapsed into the space previously occupied by the axon. These results suggest that myelin degeneration is secondary to the axonal lesion. Finally, the concentration of copper in the liver was determined using atomic absorption spectrophotometry and was found to be low (ranging from 2.2 to 10.8 ppm). In conclusion, in the Brazilian semiarid region, Cu deficiency occurs in 21 to 90-day-old pigs that ingested different types of waste in their food. MenosAbstract: The epidemiological, clinic and morphological (pathological and ultrastructural) aspects of four outbreaks of copper deficiency affecting 21- to 90-day-old pigs in the Northeast region of Brazil are reported. Clinical signs began with paraparesis and ataxia and progressed to flaccid or spastic paralysis of the pelvic and thoracic limbs, followed by sternal and/or lateral recumbence. In addition, some animals showed dog-sitting position and intention tremors. The clinical manifestation period was 5-20 days. Significant gross lesions were not observed; however, microscopically, symmetrical degeneration of the white matter with ballooned myelin sheaths containing occasional macrophages was observed, mainly in the spinal cord. Two pigs presented with necrosis ad loss of Purkinje cells and ectopic Purkinje cells in the granular layer and cerebellar white matter. A ultrastructural analysis showed different degrees of damage of myelinated axons in the spinal segments, including an absence of the axoplasm structures with only axonal residues remaining. The myelin sheaths were degenerated and often collapsed into the space previously occupied by the axon. These results suggest that myelin degeneration is secondary to the axonal lesion. Finally, the concentration of copper in the liver was determined using atomic absorption spectrophotometry and was found to be low (ranging from 2.2 to 10.8 ppm). In conclusion, in the Brazilian semiarid region, Cu deficiency occurs in 21 to ... Presentar Todo |
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Palabras claves : |
Axonal degeneration; COPPER DEFICIENCY; DEFICIENCIA DE COBRE; Degeneração axonal; Degeneração da mielina; Degenerative myelopathy; Mielopatia; MINERAL DEFICIENCY; Myelin degeneration; PLATAFORMA SALUD ANIMAL; SWINE. |
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Thesagro : |
DEFICIENCIAS DE MINERALES; ENFERMEDADES DE LOS ANIMALES; SUINOS. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/8985/1/Pesq.-Vet.-Bras.-379-911-915-setembro-2017-Olinda.pdf
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Marc : |
LEADER 02918naa a2200397 a 4500
001 1058315
005 2019-10-09
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-736X2017000900002$2DOI
100 1 $aOLINDA, R. G.
245 $aDegenerative axonopathy associated with copper deficiency in pigs. (Axonopatia degenerativa associada com deficiência de cobre em suínos).$h[electronic resource]
260 $c2017
500 $aArticle history: 1 Received on May 29, 2016 // Accepted for publication on November 11, 2016.
520 $aAbstract: The epidemiological, clinic and morphological (pathological and ultrastructural) aspects of four outbreaks of copper deficiency affecting 21- to 90-day-old pigs in the Northeast region of Brazil are reported. Clinical signs began with paraparesis and ataxia and progressed to flaccid or spastic paralysis of the pelvic and thoracic limbs, followed by sternal and/or lateral recumbence. In addition, some animals showed dog-sitting position and intention tremors. The clinical manifestation period was 5-20 days. Significant gross lesions were not observed; however, microscopically, symmetrical degeneration of the white matter with ballooned myelin sheaths containing occasional macrophages was observed, mainly in the spinal cord. Two pigs presented with necrosis ad loss of Purkinje cells and ectopic Purkinje cells in the granular layer and cerebellar white matter. A ultrastructural analysis showed different degrees of damage of myelinated axons in the spinal segments, including an absence of the axoplasm structures with only axonal residues remaining. The myelin sheaths were degenerated and often collapsed into the space previously occupied by the axon. These results suggest that myelin degeneration is secondary to the axonal lesion. Finally, the concentration of copper in the liver was determined using atomic absorption spectrophotometry and was found to be low (ranging from 2.2 to 10.8 ppm). In conclusion, in the Brazilian semiarid region, Cu deficiency occurs in 21 to 90-day-old pigs that ingested different types of waste in their food.
650 $aDEFICIENCIAS DE MINERALES
650 $aENFERMEDADES DE LOS ANIMALES
650 $aSUINOS
653 $aAxonal degeneration
653 $aCOPPER DEFICIENCY
653 $aDEFICIENCIA DE COBRE
653 $aDegeneração axonal
653 $aDegeneração da mielina
653 $aDegenerative myelopathy
653 $aMielopatia
653 $aMINERAL DEFICIENCY
653 $aMyelin degeneration
653 $aPLATAFORMA SALUD ANIMAL
653 $aSWINE
700 1 $aMAIA, L. A.
700 1 $aFRADE, M. T. S.
700 1 $aSOARES, M. P.
700 1 $aBARROS, S. S.
700 1 $aDRIEMEIER, D.
700 1 $aRIET-CORREA, F.
700 1 $aDANTAS, A. F. M.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 9, p. 911-915, setembro. 2017.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
25/06/2021 |
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Actualizado : |
25/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FEDERICI, M.; ARTIGAS, R.; GUERRA, S.; BRANDA, A.; VÁZQUEZ, N.; NICOLINI, P.; DUTRA, F.; LLAMBÍ, S. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODY ARTIGAS, Facultad de Veterinaria, Universidad de la República (UDELAR), Montevideo, Uruguay; SOFÍA GUERRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NOELIA VÁZQUEZ, Facultad de Veterinaria, Universidad de la República (UDELAR), Montevideo, Uruguay.; PAULA NICOLINI, Instituto Superior de la Carne, Universidad de la República (UDELAR), Tacuarembó, Uruguay.; FERNANDO DUTRA QUINTELA, División Laboratorios Veterinarios (DILAVE), Ministerio de Agricultura y Pesca (MGAP), Treinta y Tres, Uruguay.; SILVIA LLAMBÍ, Facultad de Veterinaria, Universidad de la República (UDELAR), Montevideo, Uruguay. |
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Título : |
Detection of the Brachyspina mutation in Uruguayan Holstein cows using real time PCR and melting curve analysis. [Detecção da mutação da Brachyspina em vacas Holandês Uruguaias usando PCR em tempo real e análise da curva de fusão.] |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Ciencia Rural, 2021, volume 51 (9), Article e20200872. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200872 |
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ISSN : |
1678-4596 (online) |
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DOI : |
10.1590/0103-8478cr20200872 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 17 Sept 2020; Accepted 05 Jan 2021; Reviewed 07 Mar 2021; Publication in this collection 02 June 2021; Date of issue 2021. |
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Contenido : |
ABSTRACT - Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed.
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RESUMO - A síndrome de Brachyspina (BS) é um defeito hereditário monogênico autossômico recessivo raro da raça Holstein Friesian causado por uma exclusão de 3,3 KB no gene FANCI localizado no cromossomo bovino 21, o que leva a um deslocamento de quadro e um códon de parada prematuro. Uma consequência da BS é a eficiência de reprodução reduzida e a produção de leite. O objetivo deste estudo foi o desenvolvimento de um método simples, rápido e sensível, baseado em PCR em tempo real e análise da curva de fusão para identificar animais portadores de BS. Sessenta e oito genótipos homozigotos normais e quatro heterozigotos foram detectados e confirmados através da análise tradicional de PCR e electophorese. Concluímos que o novo método é uma ferramenta confiável, altamente precisa e de baixo custo, que poderia ser usado para monitorar a presença da mutação BS na raça Holandês uruguaia. MenosABSTRACT - Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed.
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RESUMO - A síndrome de Brachyspina (BS) é um defeito hereditário monogênico autossômico recessivo raro da raça Holstein Friesian causado por uma exclusão de 3,3 KB no gene FANCI localizado no cromossomo bovino 21, o que leva a um deslocamento de quadro e um códon de parada prematuro. Uma consequência da BS é a eficiência de reprodução reduzida e a produção de leite. O objetivo deste estudo foi o desenvolvimento de um método simples, rápido e sensível, baseado em PCR em tempo real e análise da curva de fusão para identificar animais portadores de BS. Sessenta e oito genótipos homozigo... Presentar Todo |
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Palabras claves : |
Análise da curva de fusão; Bos taurus; Brachyspina syndrome; Holandês; Holstein; Melting curve analysis; PCR em tempo real; Real time PCR; Sindrome de Brachyspina. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://www.scielo.br/j/cr/a/kTxvzMF47Cqdb7TbPKHjxxc/?lang=en&format=pdf
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Marc : |
LEADER 03178naa a2200349 a 4500
001 1062156
005 2021-06-25
008 2021 bl uuuu u00u1 u #d
022 $a1678-4596 (online)
024 7 $a10.1590/0103-8478cr20200872$2DOI
100 1 $aFEDERICI, M.
245 $aDetection of the Brachyspina mutation in Uruguayan Holstein cows using real time PCR and melting curve analysis. [Detecção da mutação da Brachyspina em vacas Holandês Uruguaias usando PCR em tempo real e análise da curva de fusão.]$h[electronic resource]
260 $c2021
500 $aArticle history: Received 17 Sept 2020; Accepted 05 Jan 2021; Reviewed 07 Mar 2021; Publication in this collection 02 June 2021; Date of issue 2021.
520 $aABSTRACT - Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed. .-.-.-.-.-.-.-.-.-.- RESUMO - A síndrome de Brachyspina (BS) é um defeito hereditário monogênico autossômico recessivo raro da raça Holstein Friesian causado por uma exclusão de 3,3 KB no gene FANCI localizado no cromossomo bovino 21, o que leva a um deslocamento de quadro e um códon de parada prematuro. Uma consequência da BS é a eficiência de reprodução reduzida e a produção de leite. O objetivo deste estudo foi o desenvolvimento de um método simples, rápido e sensível, baseado em PCR em tempo real e análise da curva de fusão para identificar animais portadores de BS. Sessenta e oito genótipos homozigotos normais e quatro heterozigotos foram detectados e confirmados através da análise tradicional de PCR e electophorese. Concluímos que o novo método é uma ferramenta confiável, altamente precisa e de baixo custo, que poderia ser usado para monitorar a presença da mutação BS na raça Holandês uruguaia.
653 $aAnálise da curva de fusão
653 $aBos taurus
653 $aBrachyspina syndrome
653 $aHolandês
653 $aHolstein
653 $aMelting curve analysis
653 $aPCR em tempo real
653 $aReal time PCR
653 $aSindrome de Brachyspina
700 1 $aARTIGAS, R.
700 1 $aGUERRA, S.
700 1 $aBRANDA, A.
700 1 $aVÁZQUEZ, N.
700 1 $aNICOLINI, P.
700 1 $aDUTRA, F.
700 1 $aLLAMBÍ, S.
773 $tCiencia Rural, 2021, volume 51 (9), Article e20200872. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200872
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